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CryoElectron Microscopy Facilities

FEI Polara and Sphera Microscopes
The Tecnai G2 Polara is a 300KV microscope equipped with a field emission gun (FEG). It has a newly developed stage that allows for examination of multiple, vitrified samples at either liquid nitrogen or liquid helium temperatures. The Sphera is a 200KV instrument equipped with a LaB6 electron gun and it uses the standard cryotransfer holders developed by Gatan, Inc. Both microscopes are completely computer controlled with the Tecnai GUI interface that runs under the Microsoft Windows 2000 operating system. Both microscopes are also equipped with FEI tomography image acquisition software as well as the Leginon image acquistion system developed by scientists at The Scripps Research Institute.

Both microscopes are available for use, however use of the Polara will be restricted to those scientists whose projects require the use of liquid helium, the higher resolution afforded by the FEG, or the greater stability of the Polara's compustage.

Training for both instruments is available. It is expected that Polara users will have prior experience doing cryomicroscopy. For infrequent Polara users sample insertion will be done by facility personnel. For training and access to the instruments contact Norm Olson.

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Produced by the Timothy S. Baker Cryoelectron Microscopy Laboratory
Main Address: Department of Chemistry and Biochemistry MC-0378,
University of California, San Diego,
9500 Gilman Dr., La Jolla, CA 92093-0378
© 2006-2008 Timothy S. Baker. Material may not be used or reproduced in any way without permission.
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Date Modified: November 30, 2007