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Microscope Magnifications and Pixel Sizes

What do these values mean and how were they calculated?

Polara, 200 keV, April 2011
(JPEG)

Nominal Film Magnifications (screen up) Calibrated Film Magnifications Calibrated Film Pixel Sizes (Å)§ Calibrated CCD Magnifications Calibrated CCD Pixel Sizes (Å)◊
140 191 784.74
660 900 166.70
2,300 3,158 47.491
3,100 4,153 36.120
3,900 5,208 28.800
4,700 6,184 24.255
9,400 8,785 7.2287 12,108 12.388
12,000 11,388 5.5762 15,271 9.8225
15,500 15,397 4.1241 19,542 7.6758
20,000 19,595 3.2407 25,454 5.8829
23,000 22,864 2.7773 30,462 4.9242
31,000 30,191 2.1032 41,099 3.6497
39,000 38,769 1.6379 52,027 2.8831
59,000 58,050 1.0939 80,518 1.8629
78,000 75,571 0.8403 105,576 1.4208
93,000 91,689 0.6926 126,164 1.1889
115,000 115,073 0.5518 156,912 0.9559
155,000 157,178 0.4040 209,518 0.7159
200,000 195,077 0.3255 260,995 0.5747
230,000 239,030 0.2656 314,457 0.4770
§  This assumes that the film is scanned on the Nikon Super Coolscan 8000ED at 4000 ppi which corresponds to a scanning step size of 6.35 microns. Values are given in four or five significant digits although the accuracy of the calibration is not that high.

◊   Pixel size on the Gatan 4K camera is 15 microns. Values are given in four or five significant digits although the accuracy is not that high.


Polara, 300 keV, April 2011
(JPEG)

Nominal Film Magnifications (screen up) Calibrated Film Magnifications Calibrated Film Pixel Sizes (Å)§ Calibrated CCD Magnifications Calibrated CCD Pixel Sizes (Å)◊
140 169 888.67
660 883 170.002
2,300 3,122 48.053
3,100 4,052 37.021
3,900 5,080 29.526
4,700 6,099 24.595
9,400 11,839 12.670
12,000 14,994 10.004
15,500 14,611 4.3461 18,673 8.0330
20,000 18,451 3.4416 25,168 5.9600
23,000 21,900 2.8996 29,795 5.0344
31,000 29,704 2.1378 39,764 3.7723
39,000 37,977 1.6721 49,867 3.0080
59,000 56,924 1.1155 76,785 1.9535
78,000 74,899 0.8478 101,542 1.4772
93,000 89,641 0.7084 118,054 1.2706
115,000 110,533 0.5745 146,501 1.0239
155,000 250,194 0.5995
200,000 190,406 0.3335 250,194 0.5995
230,000 224,268 0.2831
§  This assumes that the film is scanned on the Nikon Super Coolscan 8000ED at 4000 ppi which corresponds to a scanning step size of 6.35 microns. Values are given in four or five significant digits although the accuracy of the calibration is not that high.

◊   Pixel size on the Gatan 4K camera is 15 microns. Values are given in four or five significant digits although the accuracy is not that high.


Sphera (August 2008)

Sphera Magnifications

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